Antifungal activity of myco-synthesized bimetallic ZnO-CuO nanoparticles against fungal plant pathogen Fusarium oxysporum

Abstract Fusarium species are considered one of the most destructing plant pathogens. In current study, bimetallic zinc oxide-copper oxide nanoparticles (ZnO-CuO NPs) were myco-synthesized using Aspergillus fumigatus for controlling oxysporum growth. was isolated from soil and identified morphologically genetically. The ZnO-CuO NPs characterized UV-Vis, DLS, HR-TEM, SEM, XRD analyses. HR-TEM characterization method indicated that, biosynthesized appeared as semi-spherical with average diameter specified 54.18 ± 1.9 nm. DLS described characteristic particle size diffusion calculated 85.52 nm, 90.85 92.85 nm ZnO NPs, CuO respectively. Additionally, SEM image displays basic NP surface character exterior impression apparent. separated naturally spherical particles connected within fungal filtrate, which illuminated fused capped filtrate. Antifungal activity evaluated against F. . Results revealed that exhibited promising antifungal toward where inhibition zone at 1000 µg/ml 22.8 0.76 mm, MIC 125 µg/ml. Moreover, growth percentages different concentrations 1000, 500, 250, 88.9, 65.5, 41.1, 8.9% respectively, highest 88.9% concentration µg/ml, while lowest TEM ultrastructure results, treated a clear destruction found in all cell contents disintegration wall well plasma membrane. Also, nucleus small damaged shape chromatin materials distributed several dark stained bodies cytoplasm. conclusion, successfully A. , it had